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N�›¤ãì'<]Ô#$%uvS€™šØ  1N  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GAGNNNNNATC,GAGNNNNNGTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        (                                                                                                                                                                      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<LastModified UTC="7:11:48">2021.6.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:11:42">2021.6.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>d00630ce-899b-455b-973b-f823c115c98f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:10:43">2021.6.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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  ?<Notes>
<UUID>d00630ce-899b-455b-973b-f823c115c98f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:10:29">2021.6.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<Type>Synthetic</Type>
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<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:10:24">2021.6.29</LastModified>
<SequenceClass>UNA</SequenceClass>
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  ><Notes>
<UUID>d00630ce-899b-455b-973b-f823c115c98f</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:10:4">2021.6.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  a      L®ZTR

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catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="4788-4794" color="#ffffff" type="standard"/><Segment range="4795-4812" color="#ffffff" type="standard"/><Segment name="-35" range="4813-4818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="2512-2560" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1834" detectionMode="exactProteinMatch"><Segment range="1769-1834" color="#cc99b2" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6762-6866" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="4570-4691" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="3204-3533" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0"><Segment range="2762-3190" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5142-5730" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="WPRE" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1858-2446" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0"><Segment range="3600-4394" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6692" detectionMode="exactProteinMatch"><Segment range="5901-6692" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="6693-6761" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FOXL1" directionality="1" translationMW="36491.39" type="CDS" allowSegmentOverlaps="0"><Segment range="701-1735" color="#ffcc00" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPGTASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDELLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="695..1579" colors="blue-black"/><ColorRange range="6891..693" colors="black-red"/><ColorRange range="694..694" colors="white"/></TopStrand><BottomStrand><ColorRange range="695..1579" colors="blue-black"/><ColorRange range="6891..693" colors="black-red"/><ColorRange range="694..694" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="694..1578" colors="blue-black"/><ColorRange range="6891..693" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="694..1578" colors="blue-black"/><ColorRange range="6891..693" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="694" val2="697"/><Node name="pEnCMV-FOXL1(human)-3Ã—FLAG-SV40-Neo.dna" type="DNA" seqLen="7086" ID="4" circular="1" resurrectable="1" operation="remove"><Features><Feature name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4768-4784" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4744-4760" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4837-4858" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="4792-4798" color="#ffffff" type="standard"/><Segment range="4799-4816" color="#ffffff" type="standard"/><Segment name="-35" range="4817-4822" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="2516-2564" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1838" detectionMode="exactProteinMatch"><Segment range="1773-1838" color="#cc99b2" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6766-6870" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="4574-4695" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="3208-3537" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0"><Segment range="2766-3194" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5146-5734" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="WPRE" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1862-2450" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0"><Segment range="3604-4398" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6696" detectionMode="exactProteinMatch"><Segment range="5905-6696" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="6697-6765" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FOXL1" directionality="1" translationMW="36491.39" type="CDS" allowSegmentOverlaps="0"><Segment range="705-1739" color="#ffcc00" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPGTASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDELLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="698..1582" colors="blue-black"/><ColorRange range="6895..693" colors="black-red"/><ColorRange range="694..697" colors="white"/></TopStrand><BottomStrand><ColorRange range="698..1582" colors="blue-black"/><ColorRange range="6895..693" colors="black-red"/><ColorRange range="694..697" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="1739..2623" colors="blue-black"/><ColorRange range="854..1738" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="1739..2623" colors="blue-black"/><ColorRange range="854..1738" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="1739" val2="1739"/><Node name="pEnCMV-MCS-3Ã—FLAG-SV40-Neo.dna" type="DNA" seqLen="7087" ID="3" circular="1" resurrectable="1" operation="remove"><Features><Feature name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4769-4785" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4745-4761" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4838-4859" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="4793-4799" color="#ffffff" type="standard"/><Segment range="4800-4817" color="#ffffff" type="standard"/><Segment name="-35" range="4818-4823" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="2517-2565" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1839" detectionMode="exactProteinMatch"><Segment range="1774-1839" color="#cc99b2" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6767-6871" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="4575-4696" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="3209-3538" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0"><Segment range="2767-3195" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5147-5735" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="WPRE" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1863-2451" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0"><Segment range="3605-4399" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6697" detectionMode="exactProteinMatch"><Segment range="5906-6697" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="6698-6766" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FOXL1" directionality="1" translationMW="36491.39" type="CDS" allowSegmentOverlaps="0"><Segment range="705-1739" color="#ffcc00" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPGTASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDELLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1740..2624" colors="blue-black"/><ColorRange range="854..1738" colors="black-red"/><ColorRange range="1739..1739" colors="white"/></TopStrand><BottomStrand><ColorRange range="1740..2624" colors="blue-black"/><ColorRange range="854..1738" colors="black-red"/><ColorRange range="1739..1739" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="1739..2623" colors="blue-black"/><ColorRange range="854..1738" colors="black-red"/></TopStrand><BottomStrand><ColorRange range="1739..2623" colors="blue-black"/><ColorRange range="854..1738" colors="black-red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="remove" val1="1739" val2="1741"/><Node name="pEnCMV-MCS-3Ã—FLAG-SV40-Neo.dna" type="DNA" seqLen="7090" ID="2" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4772-4788" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4748-4764" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4841-4862" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="4796-4802" color="#ffffff" type="standard"/><Segment range="4803-4820" color="#ffffff" type="standard"/><Segment name="-35" range="4821-4826" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="2520-2568" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1842" detectionMode="exactProteinMatch"><Segment range="1777-1842" color="#cc99b2" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6770-6874" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="4578-4699" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="3212-3541" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0"><Segment range="2770-3198" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5150-5738" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="WPRE" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1866-2454" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0"><Segment range="3608-4402" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6700" detectionMode="exactProteinMatch"><Segment range="5909-6700" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="6701-6769" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FOXL1" directionality="1" translationMW="36491.39" type="CDS" allowSegmentOverlaps="0"><Segment range="705-1742" color="#ffcc00" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPGTASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDELLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ*"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features><HistoryColors><StrandColors type="Input"><TopStrand><ColorRange range="1742..2627" colors="blue-black"/><ColorRange range="853..1738" colors="black-red"/><ColorRange range="1739..1741" colors="white"/></TopStrand><BottomStrand><ColorRange range="1742..2627" colors="blue-black"/><ColorRange range="853..1738" colors="black-red"/><ColorRange range="1739..1741" colors="white"/></BottomStrand></StrandColors><StrandColors type="Product"><TopStrand><ColorRange range="698..703" colors="red"/></TopStrand><BottomStrand><ColorRange range="698..703" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="698"/><Node name="pEnCMV-MCS-3Ã—FLAG-SV40-Neo.dna" type="DNA" seqLen="7084" ID="1" circular="1" resurrectable="1" operation="insert" hideHistory="1"><Features><Feature name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4766-4782" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4742-4758" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4835-4856" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="4790-4796" color="#ffffff" type="standard"/><Segment range="4797-4814" color="#ffffff" type="standard"/><Segment name="-35" range="4815-4820" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="2514-2562" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1836" detectionMode="exactProteinMatch"><Segment range="1771-1836" color="#cc99b2" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="6764-6868" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="4572-4693" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="3206-3535" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0"><Segment range="2764-3192" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5144-5732" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="WPRE" type="misc_feature" allowSegmentOverlaps="0"><Segment range="1860-2448" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0"><Segment range="3602-4396" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6694" detectionMode="exactProteinMatch"><Segment range="5903-6694" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="6695-6763" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="FOXL1" directionality="1" type="CDS" allowSegmentOverlaps="0"><Segment range="699-1736" color="#ffcc00" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHLFDPRLPALAASPMLYLYGPERPGLPLAFAPAAALAASGRAETPQKPPYSYIALIAMAIQDAPEQRVTLNGIYQFIMDRFPFYHDNRQGWQNSIRHNLSLNDCFVKVPREKGRPGKGSYWTLDPRCLDMFENGNYRRRKRKPKPGPGAPEAKRPRAETHQRSAEAQPEAGSGAGGSGPAISRLQAAPAGPSPLLDGPSPPAPLHWPGTASPNEDAGDAAQGAAAVAVGQAARTGDGPGSPLRPASRSSPKSSDKSKSFSIDSILAGKQGQKPPSGDELLGGAKPGPGGRLGASLLAASSSLRPPFNASLMLDPHVQGGFYQLGIPFLSYFPLQVPDTVLHFQ*"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features><HistoryColors><StrandColors type="Product"><TopStrand><ColorRange range="698..1735" colors="red"/></TopStrand><BottomStrand><ColorRange range="698..1735" colors="red"/></BottomStrand></StrandColors></HistoryColors><InputSummary manipulation="insertAt" val1="698"/><Node name="pEnCMV-MCS-3Ã—FLAG-SV40-Neo.dna" type="DNA" seqLen="6046" ID="0" circular="1" resurrectable="1" operation="invalid"><Features><Feature name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3728-3744" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3704-3720" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="3797-3818" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment name="-10" range="3752-3758" color="#ffffff" type="standard"/><Segment range="3759-3776" color="#ffffff" type="standard"/><Segment name="-35" range="3777-3782" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="1476-1524" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="798" detectionMode="exactProteinMatch"><Segment range="733-798" color="#cc99b2" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="5726-5830" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0"><Segment range="3534-3655" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0"><Segment range="2168-2497" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0"><Segment range="1726-2154" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0"><Segment range="4106-4694" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature name="WPRE" type="misc_feature" allowSegmentOverlaps="0"><Segment range="822-1410" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0"><Segment range="2564-3358" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5656" detectionMode="exactProteinMatch"><Segment range="4865-5656" color="#ccffcc" type="standard"/><Segment name="signal sequence" range="5657-5725" color="#ccffcc" type="standard"/><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features></Node></Node></Node></Node></Node></Node></Node></HistoryTree>
  Z<AlignableSequences><Sequence name="75_CMV-F_TSS20210716-027-06338_E12" ID="0" use="0" sortOrder="0"/><Sequence name="75_WPRE-R_TSS20210716-027-06338_C12" ID="1" use="0" sortOrder="1"/><Sequence name="76_CMV-F_TSS20210716-027-06338_A11" ID="2" use="0" sortOrder="2"/><Sequence name="75_CMV-F_TSS20210719-027-07229_G06" ID="3" sortOrder="3"/><Sequence name="75_WPRE-R_TSS20210719-027-07229_E12" ID="4" sortOrder="4"/><Sequence name="76_CMV-F_TSS20210719-027-07229_H06" ID="5" use="0" sortOrder="5"/><Sequence name="76_WPRE-R_TSS20210719-027-07229_F12" ID="6" use="0" sortOrder="6"/></AlignableSequences>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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  ?<Notes>
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<Type>Synthetic</Type>
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<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="1:40:59">2021.7.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                    HO  d              E              ÿÿÿÿYô?                                                                                                                                                                „<?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="EcoRI"/></CustomEnzymeSets>
